Peptide Dosing Masterclass

Michael Carroll

Apr 7

Updated: Apr 15

Important to know -

Warning, the amount of liquid in your vial is not the same as the amount of the main ingredient. In order to draw an accurate dose into your syringe, you first need to know three things.

1. You need to know the amount of solution that was mixed into your vial. If it wasn't you and you can't contact the source who mixed it for you, it will be the amount of liquid in your vial. You can take a 5ml syring and more than likely be able ot measure it.
2. You need to know the weight amount of the ingredient you are dosing that your vial originally held, (usually measured in mg).
3. You need to know the amount of the ingredient you are wanting to have in your shot. This may be confusing to you if you are focused on the amount of solution you are drawing into th syringe, you may mistakenly assume that the amount of solution in your needle matches the amount of the ingredient you are dosing, which it does not. If you don't know the amount you're supposed to dose we have an extensive library providing all of this information here.

I'm going to do the best I can to help you understand exactly how this works, because it is very important you take the proper dose. To make things easy, I'm going to do a mock dose, so just follow along with that, then after getting a good grasp on how it works you should be set to do your own dose.

The powder in your vial contains an exact amount of the ingredient you are taking. For example, Semaglutide 5mg contains 5mg of the Peptide Semaglutide before the powder is mixed into the solution. Semaglutide 5mg is the Peptide I'll be using for my mock dose so the first number we need to identify is 5.

The mixing solution is pure water that constitutes your peptide into a liquid that you can then use to administer the peptide as an injection into your body. The waters main purpose is to take your peptide into your body safely, and for the most part the amount of solution mixed into your vial does not effect the way your ingredient works. However, in order to accurately draw your desired dose, you need to know exactly how much solution was mixed into your vial. For this guide we will draw 1 ml of water to mix into the semaglutide vial. So remember that number, we will need that in a second to do the math .

In order to determine your dose you need to know the concentration of your peptide, which its the amount of peptide (mg) that's in 1ml of mixing solution.

Step 1 -

Draw the mixing solution into a syringe using a whole number like 1, 2 or 3 . You likely wont need more than that, unless you do. Either way, keep the amount a whole number so that your math is clean and simple.

Step 2 -

Mix the solution into your vial and slowly rotate the vial left and right using your hands, do this for 30 seconds.

Step 3 is the math, but before you jump to the next step, take a second to visualize our setup below so that you can get a grasp of how the math actually works. Here are the three numbers I need to know in order to draw an accurate dose.

Syringe with 1ml of bacteriostatic water.

We mixed 1 ml (or 100 units) of bacteriostatic

water into my semaglutide vial.

Semaglutide 5ml

We mixed the solution into a 5mg vial of Semaglutide.

Semaglutide 5ml

This is an example of Semaglutide that has been mixed with the concentration 5mg/ml.

The dose of Semaglutide I am wanting to take is .5mg, which ends up being pretty easy to calculate. As you can see, If I want to administer .5mg of semaglutide, then I would need to draw .1 ml (or 10 units) of liquid into the syringe . This makes it super convenient because there are exactly ten .5mg doses in 5 mg, (.5 x 10). So when i add these ten doses into 1ml of water, all I would have to do is..... that's right divide 1 by 10 (which is 0.1). So to get a .5mg dose I would draw .1 into my syringe.

So if you got it figured out naturally and you dont want to nerd out and learn the formula you should be good. But if you have a couple more minutes and want to understand the math process more exactly, lets go to step 3 and put more language to the math formula.

Step 3

The math is a two step process and we already worked it out above. But now youre going to learn the exact formula you can use for the more difficult numbers that arent as easy as my semaglutide 5mg was. We were able to do the math in our head because of how easy the math was, but when youre mixing 1 ml into 6 mg and only wanting a dose of .2 mg, then your math gets more complicated, and this is when this formula comes in handy. I'm going to work the formula still using my Semaglutide 5mg vial with a concetration of 5mg/ml and a desired dose of .5mg.

Step 1

Calculate the peptides "concetration" per 1 ml. If you drew 1ml of the liquid peptide, then

the amount of peptide in that syringe would be the number we are looking for in this step.

Formula to discover concentration in mixture..

Now lets plug in our numbers from our semaglutide example: 5mg ÷ 1mL = 5mg per mL

Step 2

Now it's time to calculate the accurate amount to draw into your syringe. This works for any dose as long as you did step 1 of the math formula correctly, which is finding the correct concentration.

To find the correct amount to draw into your syringe, you need to divide the dose you are supposed to be taking and divide that number by the concentration (Value per mL) that you discovered in step 1 above. Let's do our practice dose and see what we get.

So it looks like if I wanted to take a 0.5mg dose of Semaglutide then would come uip with this formula- 0.5mg ÷ 5mg/mL = 0.1mL.

So the final answer which is the number this whole formula was attempting to find is .1mL of solution which contains the .5 mg dose I want to take because it has a concetration of carrying 5mg for every 1mL. Notice that the illustration breaking up the syringe into mini doses earlier in this guide ended up being correct.

If you are still struggling with the concept of the math, you may be a visually enhanced learner, and you're not seeing the principle in the design. Here is a visualization of how the concentration of our Semaglutide works when dividing it a couple different ways.

Here is a model for the vial that is full with the 1ml of mixing solution mixed into a 5mg vial of Semaglutide.

Now here is a model of how it would look if you had two different size doses using the same exact vial.

The top version is what it would look like if you were taking a 1mg dose of Semaglutide out of the vial we used as practice.

The bottom version is what our practice dose looks like in a different visual.

There are many ways you can go about finding this number, butI have found this 3 step process works every time and gets easier to do the more you do it. If you have struggled with this formula before dont beat yourself up, depending on your stress or energy level it can really be a brain twister at times. But I am hoping that it makes a lot more sense now and you're able to use it to get the accurate dose amount you need to get without taking too much or too little! Oh and yeah if you didn't know we have a conversion calculator that will do the math for you. in three seconds. ;)